The red alga Chondria crassicaulis has a wide‐ranging bioactive chemical composition and is used as a local foodstuff, representing a potentially new cultivar in Korea. The cultivation techniques were developed by examining the monthly changes in frond weight in a field population of C. crassicaulis from November 2016 to October 2017. For seedling production, temperature and irradiance effects on the attachment and growth of vegetative propagules of C. crassicaulis were evaluated. In addition, effects of day length and salinity on the propagule growth were examined. C. crassicaulis is a year‐round species with a maximum frond wet weight of 817 mg observed in July 2017, as seawater temperature increases to 20°C. The attachment of vegetative propagules was significantly affected by temperature and irradiance, with maximal values detected at 20–25°C and 60 µmol photons m?2 s?1. The relative growth rates of vegetative propagules of C. crassicaulis were the highest at 20–25°C, 60 µmol photons m?2 s?1, and a salinity of 25 psu. In conclusion, due to its tremendous tolerance under variable environmental conditions, the vegetative propagules of C. crassicaulis can be used as seedlings for mass cultivation. 相似文献
AIM: To investigate the pathogenesis of chronic obstructive disease (COPD) in mice by using nasal drip of cigarette dust particles (DSP) induced pulmonary function damage model.METHODS: BALB/c mice were randomly divided into control group, LPS 100 mg/L group, DSP 0.75 mL/L group, DSP 1.5 mL/L group and DSP 3 mL/L group for 30 days. The method of nasal drip was used for 30 days to establish the COPD model. Rrs, Ers, Crs, Est, Cst, P-3/8Rn, P-3/8G and P-3/8H were measured for evaluating lung function of the mice in each group by the method of FlexiVent. The effect on the increase of airway resistance induced by methacholine (Mach) was determined using main bronchial rings by Myograph method. The HE, Masson and Resorcinol fuchsin staining of mouse tracheas and lung tissues were conducted. RESULTS: Continuous nasal drip with DSP for 30 days increased Rrs, Ers, Est, P-3/8Rn and decreased Crs, Cst, P-3/8G and P-3/8H in the mice. DSP significantly shifted the dose-effect curve of tracheal contraction induced by Mach to the left, increased the sensitivity of the airway to Mach, and significantly increased the maximal contractile airway effect of Mach. Exposure to DSP caused fibrosis of airway subepithelial, deposition of collagen in the airway basement membrane under the reticular plate, induced reticular plate thickening, pulmonary bronchial lumen serious deformation, and the inflammatory cell infiltration in the lung of mice. Significantly increased alveolar wall muscle fibers and collagen fibers were also observed. CONCLUSION: The lung function and pathomorphological changes of COPD mice induced by 30 days nasal drip of cigarette dust particles were similar to those of human COPD. 相似文献
Arbuscular mycorrhizal-like fungi (AM-like fungi) are crucial for ecosystem functioning and soil organic matter (SOM) is an indicator of soil quality. However, the spatial distribution of arbuscular mycorrhizal-like fungi, glomalin-related soil protein (GRSP) and SOM in a large scale is still unclear. The objectives of this study were to investigate the spatial distribution of SOM, arbuscular mycorrhizal-like fungi and GRSP, and reveal the potential relationship among them in a large scale across China.
Materials and methods
Soil samples (different in vegetation type, climate, and soil variables) were collected from 26 sites in a large scale across China. The soil properties including pH, total carbon (TC), total nitrogen (TN), and SOM were determined. Quantitative PCR amplification of the 18S rRNA gene was conducted to evaluate the abundance of arbuscular mycorrhizal-like fungi. The contents of easily extractable GRSP (EE-GRSP), difficultly extractable GRSP (DE-GRSP), and total GRSP (T-GRSP) were measured.
Results and discussion
Arbuscular mycorrhizal-like fungi abundance was significantly affected by the vegetation type and dramatically correlated with the soil TN and mean annual precipitation (MAP). EE-GRSP and DE-GRSP were more associated with the TC and TN content, respectively. The abundance of arbuscular mycorrhizal-like fungi significantly but weakly correlated with the T-GRSP and EE-GRSP. The SOM content positively correlated with the DE-GRSP and T-GRSP. Those results suggested that the arbuscular mycorrhizal-like fungi are a larger contributor to regulating the content of GRSP, which is an important indicator of the soil organic carbon pool.
Conclusions
Our results indicated that arbuscular mycorrhizal-like fungi abundance has a greater contribution to driving the distribution of soil C and N in a large scale by affecting the content of glomalin-related soil protein.
An enriched environment is widely used to improve domestic animals’ welfare and promote their natural behaviors. Music can reduce abnormal behavior in humans, nonhuman primates, and rodents. However, little is known about the effects of music on pigs. This study aims to explore the effects of repeated music stimulation on the behavior, physiology, and immunity of growing pigs. A total of 72 hybrid piglets (Large White × Duroc × Minpig) were randomly divided into three groups, including music (Mozart K.448, 60 to 70 dB), noise (recorded mechanical noise, 80 to 85 dB), and control (natural background sound, <40 dB), and 6 h sound stimulation was given per day (1000 to 1600 hours) from 40 to 100 d of age. The behavioral activities of the pigs were observed during the music stimulation, and their serum cortisol, salivary cortisol, and serum immune indices were also measured. Compared with the control group, the music group and noise group increased activity but decreased lying of pigs (P < 0.05). A significant increase in tail-wagging, playing, and exploring behaviors of pigs was found in the music group (P < 0.05), and the noise significantly increased the aggressive behavior of the pigs (P < 0.05). Tail-wagging, playing, exploring, manipulating, and aggressive behaviors decreased over time. Short-term (8 d) music stimulus had a lower cortisol level than that of the noise and control groups (P < 0.05), whereas long-term (60 d) music stimulus increased immunoglobulin G (IgG), interleukin-2 (IL-2), and interferon-gamma (IFN-γ) levels (P < 0.05) and decreased interleukin-4 (IL-4) level (P < 0.05). Long-term noise stimulus significantly reduced the level of IgG (P < 0.05) but did not affect the level of IL-2, IL-4, and IFN-γ levels (P > 0.05). In conclusion, short-term music stimulus (8 d) reduced the stress response, whereas long-term music stimulus (60 d) enhanced the immune responses. In addition, the noise increased the aggressive behavior, and long-term noise reduced the immunity of the growing pigs. 相似文献
Journal of Soils and Sediments - Carbon capture and storage (CCS) has been frequently discussed as a strategy for meeting CO2 emission reduction and its targets. However, some critical issues have... 相似文献